histone h3 asym dimethyl arg2 antibody Search Results


h3r2me2a  (Novus Biologicals)
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Novus Biologicals h3r2me2a
FBXW17/protein arginine N-methyltransferase 6 (PRMT6) signaling involves in cigarette smoke extract (CSE)-induced lung epithelial inflammation and apoptosis. (A) Vector, FBXW17-FLAG plasmids, scramble small hairpin RNA (shRNA), and FBXW17 shRNA were transfected into MLE12 cells separately. Annexin V and PI kits were used to detect the rate of apoptotic cells. Before detection, cells were treated with CSE for 6 h. The “percentage of cells ± SD” values were shown in all the quadrants of flow cytometry. (B) The plotted data of apoptosis in each group are presented. (C) MLE12 cells were transfected with either overexpressed or knockdown plasmids of FBXW17. Before collection, the cells were treated with 5% CSE for 6 h. Cell lysate was conducted with immunoblotting with indicated antibodies. (D) The plotted PRMT6, <t>H3R2me2a,</t> and Bcl-2 protein expression were shown. (E) The relative protein expression of H3K4me3, Bax, TNF-α, IL-1β, and COX-2 were presented. Data were represented by n = 3 separate experiments. The graph shows mean ± SD. “ ∗ ” denotes p < 0.05 between indicated groups.
H3r2me2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FBXW17/protein arginine N-methyltransferase 6 (PRMT6) signaling involves in cigarette smoke extract (CSE)-induced lung epithelial inflammation and apoptosis. (A) Vector, FBXW17-FLAG plasmids, scramble small hairpin RNA (shRNA), and FBXW17 shRNA were transfected into MLE12 cells separately. Annexin V and PI kits were used to detect the rate of apoptotic cells. Before detection, cells were treated with CSE for 6 h. The “percentage of cells ± SD” values were shown in all the quadrants of flow cytometry. (B) The plotted data of apoptosis in each group are presented. (C) MLE12 cells were transfected with either overexpressed or knockdown plasmids of FBXW17. Before collection, the cells were treated with 5% CSE for 6 h. Cell lysate was conducted with immunoblotting with indicated antibodies. (D) The plotted PRMT6, H3R2me2a, and Bcl-2 protein expression were shown. (E) The relative protein expression of H3K4me3, Bax, TNF-α, IL-1β, and COX-2 were presented. Data were represented by n = 3 separate experiments. The graph shows mean ± SD. “ ∗ ” denotes p < 0.05 between indicated groups.

Journal: Frontiers in Cell and Developmental Biology

Article Title: F-Box Protein FBXW17-Mediated Proteasomal Degradation of Protein Methyltransferase PRMT6 Exaggerates CSE-Induced Lung Epithelial Inflammation and Apoptosis

doi: 10.3389/fcell.2021.599020

Figure Lengend Snippet: FBXW17/protein arginine N-methyltransferase 6 (PRMT6) signaling involves in cigarette smoke extract (CSE)-induced lung epithelial inflammation and apoptosis. (A) Vector, FBXW17-FLAG plasmids, scramble small hairpin RNA (shRNA), and FBXW17 shRNA were transfected into MLE12 cells separately. Annexin V and PI kits were used to detect the rate of apoptotic cells. Before detection, cells were treated with CSE for 6 h. The “percentage of cells ± SD” values were shown in all the quadrants of flow cytometry. (B) The plotted data of apoptosis in each group are presented. (C) MLE12 cells were transfected with either overexpressed or knockdown plasmids of FBXW17. Before collection, the cells were treated with 5% CSE for 6 h. Cell lysate was conducted with immunoblotting with indicated antibodies. (D) The plotted PRMT6, H3R2me2a, and Bcl-2 protein expression were shown. (E) The relative protein expression of H3K4me3, Bax, TNF-α, IL-1β, and COX-2 were presented. Data were represented by n = 3 separate experiments. The graph shows mean ± SD. “ ∗ ” denotes p < 0.05 between indicated groups.

Article Snippet: H3R2me2a (catalog no. NB21-1002) was purchased from Novus Biologicals (Littleton, CO, United States).

Techniques: Plasmid Preparation, shRNA, Transfection, Flow Cytometry, Knockdown, Western Blot, Expressing